TABLE 1.

Determination of INH derivatives produced after nonenzymatic activation in the absence or presence of nicotinamide or nicotinic coenzymes

Run no.	Coenzyme (2 mM)	Mn (III) pyrophosphate concn (mM)	INH concn	% INH converted after 15 min	% INH derivatives yielda			% INH adduct yielda (pool)
					1	2	3
1		1	500 μM	93	45	12	4
2			500 μM	<3b	0.1	<1	<1
3	NAD+	4	2 mM	60	10	5	NDc	41
4	NADH	4	2 mM	53	16	3	ND	14
5	NMN+	4	2 mM	60	14	8	ND	5
6	DNAD+	4	2 mM	84	12	7	ND	6

^aBased on initial INH concentration after 20 min incubation.

^bNo evolution even when incubated for 48 h.

^cND, not determined.