TABLE 2.

Inhibition of InhA activity by adducts formed in the presence of InhA or by preformed adducts (INH, 100 μM; InhA, 3 μM)

Run no.	Coenzyme (100 μM)	Mn (III) pyrophosphate concn of 200 μM	Preincubation time (min before InhA addition)	% InhA activity remaining after:
				5 min	12 min
1	NADH	+	0	43 ± 4	21 ± 2
2	NADH	−	0	90 ± 12	79 ± 18
3		+	0	86 ± 11	69 ± 7
4	NADH	+	18	65 ± 10	40 ± 13
5		+	18	102 ± 11	86 ± 8
6	NADH	−	18	116 ± 19	104 ± 6
7	NAD+	+	18	8 ± 4	10 ± 3
8	NAD+	+	6	20	13
9	NAD+	+	2	14	14
10	NAD+	−	18	92 ± 6	89 ± 16
11	NMN+	+	18	99	106
12	NMN+	−	18	105	100
13	DNAD+	+	18	100	94
14	DNAD+	−	18	100	97