TABLE 3.
Transformation of pneumococcal strain R6 with DNA from isolate 72521a
| Recipient | Transforming DNA | MIC (μg/ml) for transformants
|
Altered pbp gene(s) in transformants | |
|---|---|---|---|---|
| Penicillin | Amoxicillin | |||
| R6 | pbp2x | 0.03 | 0.03 | pbp2x |
| R6 | pbp2x and pbp1a | 0.03 | 0.03 | pbp2x |
| R672521/2x | pbp2b | 0.12 | 0.25 | pbp2x, pbp2b |
| R672521/2x/2b | pbp1a | 0.5 | 1 | pbp2x, pbp2b, pbp1a |
| R672521/2x/2b/1a | pbp1b, pbp2a, and pbp3 | —b | — | |
| R672521/2x/2b/1a | genomic DNA | 4 | 8 | pbp2x, pbp2b, pbp1a |
| R672521/2x/2b/1a | murMN | — | — | |
| R63191/2x/2b/1a | murMN | 12 | 8 | pbp2x, pbp2b, pbp1a, MurMN |
a
Penicillin and amoxicillin were used as selective antibiotics.
b
—, no transformants were selected.