TABLE 3.
Sensitivity of drug-resistant strains of HCMV to benzimidazole ribosides and GCV, as determined by a DNA hybridization assay
| Virus strain | Mean IC50 ± SEM (μM)a
|
Altered gene | Amino acid change (s) | ||
|---|---|---|---|---|---|
| 1263W94 | GCV | BDCRB | |||
| AD169 | 0.08 ± 0.02 | 0.31 ± 0.1 | 0.7 ± 0.3 | NAb | NA |
| 8805rec1 | 0.03 ± 0.01 | 1.7 ± 0.1 | 2 ± 1 | UL97 | Met460Val |
| 9330rec5 | 0.13 ± 0.06 | 1.6 ± 0.4 | 0.7 ± 0.3 | UL97 | His520Glu |
| 8702rec2 | NDd | 1.8 ± 0.3 | 0.75 ± 0.3 | UL97 | Ala594Val |
| 9219rec7 | 0.2 ± 0.1 | 1.8 ± 0.3 | 1 ± 0.8 | UL97 | Leu595del |
| XbaF.4 | 0.03 ± 0.01 | 4.1 ± 0.9 | 0.2 ± 0.2 | UL97 | AlaAlaCysArg593del |
| 4955rec | 0.06 ± 0.03 | 0.7 ± 0.07 | ND | polc | Thr700Ala |
| 1038rB | 0.04 ± 0.02 | 0.24 ± 0.06 | >20 | UL89 | Asp344Glu and Ala355Val |
| 2916rAe | 2.7 ± 0.6 | 0.31 ± 0.04 | 0.48 ± 0.12 | UL97 | Leu397Arg |
a
That is, the mean values based on a minimum of three independent determinations.
b
NA, not applicable.
c
pol, polymerase.
d
ND, not determined.
e
DNA sequencing of the entire virus was not performed. The sequence change in the UL97 ORF conferred resistance to 1263W94 when transferred into wild-type strain AD169.