TABLE 1.
Oligonucleotide primers and annealing temperatures (Ta) used in this study
| Gene | Primera | Ta (°C) | Gene fragmentb |
|---|---|---|---|
| gyrA | 5′ CCAGATGT(A/C/T)CG(A/C/T)GATGG (F) | ||
| 5′ ACGAAATCAAC(G/C)GT(C/T)TCTTTTTC (R) | 52 | 103-438 | |
| gyrB | 5′ TGA(C/T)GATGC(G/C/A)CG(T/C)GAAGG (F) | ||
| 5′ CGTACG(A/G)ATGTG(C/A)GA(G/A)CC (R) | 54 | 936-1506 | |
| 5′ CCACATCCGTCATGATAA (S) | 55 | 1497c | |
| parC | 5′ TTGCC(A/T)TTTAT(C/T)GG(G/T)GATGG (F) | ||
| 5′ CGCGC(A/T)GGCAGCATTTT(A/T)GG (R) | 52 | 91-583 | |
| parE | 5′ GCA(G/A)GA(T/G)(C/G)CGCA(G/A)TT(T/C)G (F) | ||
| 5′ ATC(A/C/G)GAGTC(C/T/G)GCATCCG (R) | 56 | 972-1466 |
a
Degenerate primers designed from alignment of known sequences. Abbreviations: F, forward; R, reverse; S, sequencing primer.
b
Nucleotide positions are based on E. coli gene sequences.