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Transmission electron micrographs of β-lactamase-negative K. pneumoniae colony biofilms from the untreated 12-h control (A to C) and exposed to 5,000 μg of ampicillin per ml for 12 h (D to F). (A and D) Spot near the air interface; (B, E1, and E2) locations near the middle of the biofilm; (C and F) region near the membrane. The interior of the ampicillin-treated biofilm contained both zones of obvious antibiotic action (E1) and little antibiotic action (E2). Scale bar, 5 μm.
