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Spatial variation in ampicillin action in a β-lactamase-negative K. pneumoniae colony biofilm visualized by TEM. The biofilm was exposed to 5,000 μg of ampicillin per ml for 12 h. These two panels are montages along a single transect. The air interface was at the right and the membrane at the left. The gap between the two panels contained a bar of the copper grid supporting the section. This gap was about 50 μm.
