TABLE 2.
Susceptibilities of isogenic mutants of P. aeruginosa PAO to penems and other antimicrobial agents
| Strain | Phenotypea
|
MIC (μg/ml) ofb:
|
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AB | XY | CD | M | J | Faropenem | Ritipenem | AMA3176 | Sulopenem | Sch29482 | Sch34343 | Norfloxacin | Tetracycline | |
| PAO1 | + | − | − | + | − | 512 | 128 | 128 | 32 | 256 | 128 | 0.5 | 8 |
| KG5002 | − | − | − | − | − | 1 | 2 | 1 | 0.125 | 0.5 | 1 | 0.063 | 1 |
| KG5004 | ++ | − | − | ++ | − | 4,096 | 256 | 1,024 | 64 | 2,048 | 1,024 | 2 | 32 |
| KG5006 | − | ++ | − | ++ | − | 4 | 8 | 8 | 0.5 | 8 | 8 | 2 | 16 |
| KG5008 | − | − | ++ | − | ++ | 16 | 4 | 64 | 2 | 32 | 32 | 16 | 32 |
| KG2504 | + | − | − | + | − | 256 | 32 | ND | 8 | 256 | ND | 0.5 | 8 |
| KG2504F1 | ++ | − | − | ++ | − | 2,048 | 128 | ND | 64 | 1,024 | ND | 2 | 32 |
| KG2505 | − | − | − | − | − | 1 | 2 | 1 | 0.125 | 1 | 1 | 0.25 | 2 |
| KG2505F1 | − | − | ++ | S | ++ | 16 | 4 | ND | 2 | 16 | ND | 8 | 16 |
| KG2505F1ΔD | − | − | − | S | − | 1 | 2 | ND | 0.125 | 1 | ND | 0.25 | 2 |
a
AB, MexAB; XY, MexXY; CD, MexCD; M, OprM; J, OprJ; +, wild-type-level expression; ++, overexpression; −, undetectable expression; S, lower, although significant, level of expression than the wild-type level.
b
Expression of the efflux systems was determined by immunoblot analysis using an antibody specific to a component of each efflux system as described in the text. ND, not done.