TABLE 1.
SSCP patterns and QRDRs of gyrA of 138 ciprofloxacin-resistant and 24 ciprofloxacin-susceptible C. jejuni isolates
| SSCP pattern | No. (%) of isolates analyzeda
|
Ciprofloxacin MIC range (μg/ml) | Nucleic acid codons and corresponding amino acids of C. jejuni QRDR of gyrAb
|
||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CIP-S | CIP-R | Codon | Amino acid | Codon | Amino acid | Codon | Amino acid | Codon | Amino acid | Codon | Amino acid | Codon | Amino acid | Codon | Amino acid | Codon | Amino acid | ||
| ICIP-S | 7 (29) | 0.064-0.5 | GGT | Gly-78 | CAC | His-81 | ACA | Thr-86 | GAT | Asp-90 | CCA | Pro-104 | GGC | Gly-110 | AGT | Ser-119 | GCC | Ala-120 | |
| ICIP-R1 | 21 (15) | 16->64 | --- | - | --- | - | -T- | Ile-86 | --- | - | --- | - | --- | - | --- | - | --- | - | |
| ICIP-R2 | 1 (0.7) | >64 | --- | - | --- | - | -T- | Ile-86 | A-- | Asn-90 | --- | - | --- | - | --- | - | --- | - | |
| ICIP-R3 | 1 (0.7) | 16 | --- | - | --- | - | -T- | Ile-86 | --- | - | T-- | Ser-104 | --- | - | --- | - | --- | - | |
| IICIP-S | 2 (8) | 0.25-0.5 | --C | - | --- | - | --- | - | --- | - | --- | - | --- | - | --C | - | --- | - | |
| IIICIP-S | 15 (63) | 0.125-1 | --- | - | --T | - | --- | - | --- | - | --- | - | --- | - | --C | - | --T | - | |
| IIICIP-R1 | 113 (82) | 8->64 | --- | - | --T | - | -T- | Ile-86 | --- | - | --- | - | --- | - | --C | - | --T | - | |
| IIICIP-R2 | 2 (1.4) | 32 | --- | - | --T | - | -T- | Ile-86 | --- | - | --- | - | --T | - | --C | - | --T | - | |
a
Number of isolates sequenced per SSCP pattern: ICIP-S, 4; ICIP-R1, 2; ICIP-R2, 1; ICIP-R3, 1; IICIP-S, 2; IIICIP-S, 9; IIICIP-R1, 12; IIICIP-R2, 2. CIP-S, ciprofloxacin susceptible (MIC, ≤1 μg/ml); CIP-R, ciprofloxacin resistant (MIC, ≥4 μg/ml).
b
-, no change.