FIG. 2.

Structures of R388 and R388::Tn1404. (A) BamHI digests of R388, R388::Tn1404, and pRM857. Size markers are SPP1 digested with EcoRI and bacteriophage λ digested with HindIII, and the sizes of the fragments (in kilobases) are indicated. (B) Maps of integron In3 in R388 and the cassette array in R388::Tn1404. The 5′-CS (thick line) is bounded at one end by IRi and ends with the attI1 site (narrow open box). Gene cassettes are shown as open boxes and an adjacent filled box representing the 59-be or simple site. The 3′-CS region present is represented by a thin line, and flanking regions are shown as dashed lines. BamHI sites (labeled B) and the sizes of the fragments expected on BamHI digestion are shown.