FIG. 4.

Alignment of cassette-encoded AadA proteins. The sequences are identified on the left by the numeral in the protein name, and more closely related sequences are grouped. The first ATG after the beginning of the cassette has been used as the start codon, although AadA1, AadA2, AadA7, and AadA9 also have a second potential start codon (GTG) which corresponds to the valine at position 5. Residues identical in seven or all eight (boldface type) sequences are indicated in the consensus sequence shown below. Residues that are also conserved in the Aad proteins from the sequences with GenBank accession nos. X68089, X02588, M69221, AF288536, AF408195, AL392149, and AE007309 are boxed. The protein sequences are translations of DNA sequences in AadA1 (GenBank accession no. X12870), AadA2 (GenBank accession no. X68227), AadA4 (GenBank accession no. Z50802.3), AadA5 (GenBank accession no. AF137361), AadA6 (GenBank accession no. AF140629), AadA7 (GenBank accession no. AF224733), and AadA9 (GenBank accession no. AX135967 or AJ420072). The AadA3 (GenBank accession no. AF047479) and AadA8 (GenBank accession no. AF326210) proteins have not been included in the analysis, as they can be considered hybrids of AadA1 and AadA2.