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. 2005 Nov;73(11):7113–7125. doi: 10.1128/IAI.73.11.7113-7125.2005

FIG. 2.

FIG. 2.

FIG. 2.

EPEC-induced cytoskeletal rearrangements are disrupted by cholesterol depletion. Immunofluorescence micrographs (a to c) using an α-actinin antibody (left column) and EPEC immune serum (middle column) and merged images (right column). (a) Uninfected cells show no cytoskeletal rearrangements and lack bacterial staining. (b) Cells infected with EPEC showed microcolony-type bacterial adherence at sites of EPEC-induced α-actinin mobilization (arrows). (c) HEp-2 cells depleted of cholesterol (10 mM MβCD; 1 h) prior to EPEC infection show a reduced number of focal cytoskeletal rearrangements at the few sites of bacterial adherence. (d) Graphical representation of semiquantification of cytoskeleton rearrangements with increasing concentrations of MβCD. The findings show a dose-dependent decrease in the formation of EPEC-induced α-actinin foci (ANOVA, P < 0.001). The error bars indicate standard errors.