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. 2005 Nov;73(11):7142–7150. doi: 10.1128/IAI.73.11.7142-7150.2005

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FIG. 3. — Dissemination of Y. pestis(pACGFP) into cells of the innate immune system following subcutaneous infection of BALB/c mice. The spleens from groups of five BALB/c mice were removed on days 1, 2, 3, 4, and 5 postinfection. Neutrophils (Ly-6G⁺ cells) and macrophages (CD11b⁺ cells) were isolated using Minimacs beads, stained for the presence of Ly-6G and CD11b, fixed in 4% paraformaldehyde for 24 h at 4°C, and analyzed by flow cytometry. (A) Dot plots from CD11b⁺ and Ly-6G⁺ populations on each day postinfection, indicating the presence of GFP fluorescence. Regions from which CD11b⁺ and Ly-6G⁺ cell data were gated are also indicated. (B) Percentages of CD11b⁺ (solid bars) and Ly-6G⁺ (open bars) cells expressing GFP over the course of infection. The data are means with 95% confidence limits.

FIG. 3. — Dissemination of Y. pestis(pACGFP) into cells of the innate immune system following subcutaneous infection of BALB/c mice. The spleens from groups of five BALB/c mice were removed on days 1, 2, 3, 4, and 5 postinfection. Neutrophils (Ly-6G⁺ cells) and macrophages (CD11b⁺ cells) were isolated using Minimacs beads, stained for the presence of Ly-6G and CD11b, fixed in 4% paraformaldehyde for 24 h at 4°C, and analyzed by flow cytometry. (A) Dot plots from CD11b⁺ and Ly-6G⁺ populations on each day postinfection, indicating the presence of GFP fluorescence. Regions from which CD11b⁺ and Ly-6G⁺ cell data were gated are also indicated. (B) Percentages of CD11b⁺ (solid bars) and Ly-6G⁺ (open bars) cells expressing GFP over the course of infection. The data are means with 95% confidence limits.