FIG. 2.

Lst sequences from 6 meningococcal and 14 gonococcal strains were aligned using ClustalW. A representative alignment of the bifunctional Lsts from meningococcal strain 126E and gonococcal strain 398078 is shown. Species-specific variation occurred at 21 separate amino acid positions (boxed) distributed across the protein. Strain variation within a species (shaded) was noted at 11 meningococcal (above) and 7 gonococcal (below) amino acid positions. The isoleucine (Ile) residue at position 168, shown to be critical for bifunctionality of 126E Lst, is marked with an asterisk.