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. 2005 Nov;73(11):7727–7735. doi: 10.1128/IAI.73.11.7727-7735.2005

FIG. 1.

FIG. 1.

In vitro levels of expression of antigen 85B mRNA from the Sin85B and pVax-85B vectors were similar. RT-PCR analysis of mRNA from RD cells transfected with Sin85B (RD-Sin85B) (lanes 2 and 3, 10 and 5 μl of the PCR product, respectively) or pVax-85B (RD-pVax-85B) (lanes 4 and 5, 5 and 10 μl of the PCR product, respectively) using antigen 85B-specific primers was performed. Using the same mRNA, RT-PCR using GAPDH-specific primers was performed (lane 7, RD-Sin85B mRNA; lane 8, RD-pVax-85B mRNA) as controls. Lanes 1 and 6 contain DNA molecular size markers.