FIG. 7.
Analysis for the presence of renal immune complex deposits of total Igs by using a direct immunofluorescence method. A direct immunofluorescence assay was performed with FITC-conjugated rabbit anti-mouse Igs incubated on a kidney cryostat section from (a) M. vaccae-primed and MTB70 (two times)-boosted mice, (b) HgCl2-treated SJL mice, and (c) nonimmunized mice. Staining of a glomerulus is seen at 63× magnification. We show here the staining with a 1:40 dilution of the FITC-conjugated rabbit anti-mouse Igs.