TABLE 2.
Plasmids used in this study
| Plasmid | Featuresa | Reference or source |
|---|---|---|
| pGY-100 | sacB CmroriR6K | 44 |
| pMMB207αb-Km-14 | IncQ lacIq CmrmobA::kan MCS | 33 |
| pUC18 | ori ColE1 MCS Apr | 40 |
| pGS-lac-02 | Promoterless lacZ incQ Cmr | 14 |
| pGY-dotD-12 | 1kb of dotD upstream region in pUC-18 | This study |
| pGY-dotD-34 | 1kb of dotD downstream region in pUC-18 | This study |
| pGY-D-Km-01 | The dotD upstream and downstream regions with the Km cassette between them in pUC-18 | This study |
| pGY100-D-Km-01 | The insert of pGY-D-Km-01 in pGY-100 | This study |
| pGY100-D-01 | pGY100-D-Km-01 without the Km cassette | This study |
| pGY-dotC-12 | 1kb of dotC upstream region in pUC-18 | This study |
| pGY-dotC-34 | 1kb of dotC downstream region in pUC-18 | This study |
| pGY-C-Km-01 | The dotC upstream and downstream regions with the Km cassette between them in pUC-18 | This study |
| pGY100-C-Km-01 | The insert of pGY-C-Km-01 in pGY-100 | This study |
| pGY100-C-01 | pGY100-C-Km-01 without the Km cassette | This study |
| pGY-CD-Km-01 | The dotD upstream and dotC downstream regions with the Km cassette between them in pUC-18 | This study |
| pGY100-DC-Km-01 | The insert of pGY-CD-Km-01 in pGY-100 | This study |
| pGY100-DC-01 | pGY100-DC-Km-01 without the Km cassette | This study |
| pGS-Le-40-Km-1 | icmN with the Km cassette | 31 |
| pGS-BCD-01 | The dotDCB region in pUC-18 | 18 |
| pGY-dotD-02 | dotD in pMMB207αb-Km-14 | This study |
| pGY-dotDC-02 | dotDC in pMMB207αb-Km-14 | This study |
| pGY-DCB-06 | dotDCB in pMMB207αb-Km-14 | This study |
| pGY-DCB-09 | dotDCB in pMMB207αb-Km-14 | This study |
| pGY-dotC-SE-01 | Part of dotC and dotB in pUC-18 | This study |
| pGY-dotDC-SB-01 | dotD and part of dotC in pUC-18 | This study |
| pGY-D-C19S-01 | dotD C19S in pUC-18 | This study |
| pGY-C-C19A-02 | dotC C19A in pUC-18 | This study |
| pGY-D-C19S-02 | pGY-DCB-09 with dotD C19S | This study |
| pGY-C-C19A-03 | pGY-DCB-09 with dotC C19A | This study |
| pGY-DC-C19SA-01 | pGY-DCB-09 with dotD C19S and dotC C19A | This study |
| pGY-dotDlac-06 | 122 bp upstream of dotD in pUC-18 | This study |
| pGY-dotDlac-07 | 122 bp upstream of dotD in pGS-lac-02 | This study |
| pSR-N(V)-lac | 444 bp upstream of icmN in pGS-lac-02 | This study |
a
MCS, multiple cloning site; Ap, ampicillin.