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. 2005 Nov;73(11):7259–7266. doi: 10.1128/IAI.73.11.7259-7266.2005

TABLE 2.

Primers used in this study

Gene and primer Sequence
iruA
    a1-1F 5′-GCG CTC GAG ATT GAG GAA TTA AGT GAT GC-3′
    a1-1R 5′-GCG GAT CCA GGA TCG TCT TTA CTC TCA G-3′
    a1-1uF 5′-ATC GCG AAA CTC ATC GAG GAG CAA-3′
    a1-1uR 5′-TTC TTT CGG CCA ACT CAG TGG CTT-3′
    a1-1dF 5′-AGC AGC TGA AGA ATC GAG AGT AGC-3′
    a1-1dR 5′-TGA GCG TGA ATC TGG ATG AAG AGA AC-3′
    a1rt-F 5′-ACA AGA GCT AAG TCT GCA GG-3′
    a1rt-R 5′-AGC AAG CTG AGA AGC ATA GG-3′
iruB
    b5-1F 5′-GCG CTC GAG AAC GAC TCA GAA CGT TTA GC-3′
    b5-1R 5′-GCG GAT CCC TTA AAT TGA AAA GTC CGT G-3′
    b5-1uF 5′-ATT CCG CCA AAT CAA CGT AGG TGC-3′
    b5-1uR 5′-ATA AGT GAC CGC TGC GTC AGG ATT-3′
    b5-1dR 5′-CAT GAT CGC CAG TTC TTG GTT GTT G-3′
    b51rt-F 5′-ACT GCG ACA AGA GCT CAA GT-3′
    b51rt-R 5′-GTT GTC TAT CGG TCC AGA TG-3′