FIG. 2.

CD8⁺ T-cell polarization. Cytokines were measured in supernatants collected 48 h after Tc1 and Tc2 CD8⁺ T-cell restimulation with either ConA (Con A) or DCs pulsed with antigen (DC/PC). A third group of cells was exposed to medium, and the cytokine concentrations obtained were subtracted from those of the stimulated samples. Cytokines levels were measured using the BioPlex system from Bio-Rad (Hercules, CA) as described in Materials and Methods. Concentrations were expressed in pg/ml. (*, P of <0.05 compared to respective control).