FIG. 3.

In vitro effector activity of Tc1 CD8⁺ T cells. Tc1 and Tc2 in vitro-polarized CD8⁺ T cells (A) purified from the spleens of P. murina-vaccinated-boosted BALB/c mice, as well as the supernatants obtained from Tc1 and Tc2 cultures (B), were incubated with P. carinii and thioglycolate-induced peritoneal macrophages for 24 h. Controls included P. murina cultured without macrophages. Thereafter, RNA was isolated from the contents of each well, and quantitative real-time PCR for P. carinii rRNA copy number was performed as described in Materials and Methods. Percent killing at 24 h was assayed by quantifying the integrity of the P. murina rRNA subunit by TaqMan in comparison to P. murina organisms cultured without macrophages (*, P < 0.05, analysis of variance [ANOVA]).