FIG. 5.

Effects of GM-CSF and IFN-γ blocking in the presence of Tc1 supernatants. Thyoglycolate-induced peritoneal macrophages were added to P. murina in the presence of Tc1 supernatant (Tc1 sup.). Neutralization of GM-CSF and IFN-γ was performed using 2 μg/ml of murine anti-GM-CSF and/or the same concentration of murine anti-IFN-γ antibodies (Biosource, Camarillo, California). Controls included P. murina cultured with macrophages in medium alone. Results are expressed as the means ± SEMs of results for triplicate samples from one representative experiment (*, P < 0.05, ANOVA).