FIG. 2.

ELISA inhibition by different fractions obtained from C. neoformans CneF. Plates were coated with CneF, and pooled plasma from cryptococcosis patients was mixed with putative inhibitors before addition to the wells. Whole CneFs from Cap 67 and B. dermatitidis supernatants were used as positive and negative controls, respectively. (A) Inhibitory effects of ConA-binding or nonbinding fractions. (B) Purification of the ConA-binding fraction into three peaks by DEAE anion-exchange chromatography. Bars indicate pooled fractions obtained using a linear gradient of NaCl (right-hand axis). (C) Inhibitory effects of the different DEAE peaks. One experiment, representative of three, each performed in duplicate, is shown.