FIG. 6.

Analysis of recombinant MP84 and MP115 proteins. SDS-PAGE was followed by Coomassie staining (A), ConA blot (B), Western blot with a pool of sera from patients (C), Western blot with a pool of normal sera from volunteers (D), Western blot with a pool of sera from infected mice (E), or Western blot with a pool of sera preinfection mice (F). All pooled sera were used at a 1:100 dilution. Purified recombinant 84-kDa and 115-kDa proteins were loaded onto lanes 1 and 2, respectively. Numbers on the left indicate the molecular masses in kilodaltons.