TABLE 1.
Oligonucleotide probes and primers used to identify the mef and orf genes
| Oligonucleotide | Sequence (5′-3′) | Genea | Reference |
|---|---|---|---|
| ORF1 | TGA TGA AGA GGA AAA TTA G | orf1 | This study |
| ORF2 | GCA TCA GGA ACA TCA ATC | orf2 | This study |
| ORF3 | GGT ATT GTT CAG GTA GGT C | orf3 | This study |
| MEFF | ATG GAA AAA TAC AAC AAT TGG AAA C | mef(A) | This study |
| MEFRa | TTA TTT TAA ATC TAA TTT TCT TAA | mef(A) | This study |
| MF4 | ACC GAT TCT ATC AGC AAA G | mef(A) | 6 |
| MF4AR | TTC TTT GCT GAT AAA ATC GGT GT | mef(A) | This study |
| MF6 | GGA CCT GCC ATT GGT GTG | mef(A) | This study |
| MF6R | GCA CAC CAA TGG CAG GTC C | mef(A) | This study |
| MF7 | ATG CAG ACC AAA AGC CAC AT | Upstream from mef(A) | 6 |
| ORF5 | CAT CTG GGT GAA CTT GCC | orf5 | This study |
| ORF5FRev | GGC AAG TTC ACC CAG ATG | orf5 | This study |
| ORF6 | CAT GTT GGA GTA GCG GTA G | orf6 | This study |
| ORF7 | GAA ATC TTT GGT CAG ACT TGG | orf7 | This study |
| ORF8 | CCC TCC AAT CCA CCA GCG | orf8 | This study |
a
Taken from Tn1207.1. All the primers except MEFF, MEFR, and MF7 are within the genes listed. MEFF is the first 25 bp of the mef(A) gene; MEFR contains the last 6 bp of the mef(A) gene and 15 bp downstream of the gene.