FIG. 2.

Immunogenicity of recombinant adenovirus expressing ANYNFTLV. C57BL/6 mice were administered with either Ad-MANY or Ad-GFP intramuscularly at three different doses (A, B) or intraperitoneally at two different doses (C, D). The mice were sacrificed 14 days after the immunization, and their spleens were removed. A half of splenocytes from individual mice were cultured with irradiated EL-4 cells pulsed with ANYNFTLV peptide for one week. The freshly isolated splenocytes (A, C) or the 1-week cultured splenocytes (B, D) were subjected to the ELISPOT assay for IFN-γ-producing cells in response to ANYNFTLV peptide-pulsed EL-4 cells. The number of IFN-γ-secreting cells/10⁶ cells was counted 24 h later. The number of IFN-γ-secreting cells that appeared against peptide-unpulsed EL-4 was subtracted from the number of IFN-γ-secreting cells that appeared against peptide-pulsed EL-4. Data represent the mean ± standard deviation of three mice in each group. The data are representative one of three independent experiments.