TABLE 2.
Supernatant neutralization with different antibodies
| Supernatant | Vegetative culture (phenotype 1)a | Epsilon antibody | Alpha antibody |
|---|---|---|---|
| 1 | PLC(4.2), PFO(5.5), ETX(53) | Yes | NDb |
| 2 | PLC(1.6), PFO(<1), ETX(17) | Yes | ND |
| 3 | PLC(2.5), PFO(<1), ETX(6) | Yes | No |
| 4 | PLC(<1), PFO(1.8), ETX(6) | Yes | ND |
| 5 | PLC(1.9), PFO(<1), ETX(25) | Yes | ND |
| 6 | PLC(2.1), PFO(<1), ETX(13) | Yes | ND |
| 7 | PLC(4.6), PFO(3.5), ETX(5) | Yes | ND |
| 8 | PLC(2.1), PFO(<1), ETX(8) | Yes | No |
| 9 | PLC(1.5), PFO(<1), ETX(10) | Yes | No |
| 10 | PLC(1.2), PFO(1.6), ETX(5) | Yes | ND |
| 11 | PLC(<1), PFO(2.7), ETX(3) | ND | ND |
| 12 | PLC(5.1), PFO(2.9), ETX(2) | Yes | ND |
| 13 | PLC(2.1), PFO(<1), ETX(8) | ND | ND |
| 14 | PLC(3.8), PFO(2.9), ETX(4) | Yes | No |
| 15 | PLC(1.1), PFO(<1), ETX(4) | Yes | No |
| 16 | PLC(<1), PFO(2.7), ETX(3) | ND | ND |
| 17 | PLC(1.4), PFO(<1), ETX(<1) | ND | ND |
| 18 | PLC(2.0), PFO(3.7), ETX(52), CPE(0) | Yes | ND |
| 19 | PLC(4.6), PFO(3.5), ETX(2), CPE(0) | ND | ND |
| 20 | PLC(2.1), PFO(<1), ETX(13) | ND | ND |
| 21 | PLC(<1), PFO(1.6), ETX(5), CPB2(0.03) | ND | ND |
| Alpha-toxin | PLC | No | Yes |
a
Values shown in subscript parentheses for ETX, CPB2, and CPE are micrograms of protein/ml and for PFO and PLC are activity levels (log2 titer or U/ml [10−3], respectively).
b
ND, not determined.