TABLE 1.
Genotypes of Candida albicans strains used in this study
| Strain | Genotype | MTL genotype | Source and/or reference |
|---|---|---|---|
| SC5314 | Wild typea | a/α | 13 |
| 3116b | Wild type | a/a | Sorbose selection from SC5314 (this work) |
| 3117b | Wild type | α/α | Sorbose selection from SC5314 (this work) |
| 4448b | Wild type | a/a | Sorbose selection from SC5314 (this work) |
| 4449b | Wild type | α/α | Sorbose selection from SC5314 (this work) |
| 4450 | Wild type | a/a/α/α | Mating product of 4448 and 4449 (this work) |
| CAI4.URA | URA3/ura3 | a/α | CAI-4 (8) |
| 3142 (CHY444) | ura3/ura3 MTLa/mtlα1::hisG mtlα2::hisG | a/Δα | 17 |
| 3153 | ura3/ura3 ade2::hisG/ade2::hisG mtla1a2::hisG/MTLα | α/Δa | Derived from CHY477 (33) (this work) |
| 3153.URA | URA3/ura3 ade2::hisG/ade2::hisG mtla1a2::hisG/MTLα | α/Δa | Derived from 3153 (this work) |
| 3832 | URA3/ura3/ura3/ura3 ADE2/ADE2/ade2/ade2 MTLa/mtlα1::hisG mtlα2::hisG MTLα/mtla1a2::hisG | a/α/Δa/Δα | Mating product of 3142 (35) and 3153.URA |
| 3834 | Same as 3832 | a/α/Δa/Δα | Mating product of 3142 (35) and 3153.URA |
| 3609 | ura3/ura3/ura3/ura3 ADE2/ADE2/ade2::hisG ade2::URA3 MTLa/mtlα1::hisG mtlα2::hisG MTLα/mtla1a2::hisG | a/α/Δa/Δα | Mating product of 3142 (35) and CHY477 (33) |
| 1035 | Wild type | a/α | FC18 (40) |
| 3567b | Wild type | a/a | Sorbose selection from FC18 (this work) |
| 3568b | Wild type | α/α | Sorbose selection from FC18 (this work) |
| 3316b | arg/arg ilv/ilv | a/a | Sorbose selection from A505 (20) (this work) |
| 3320b | trp1/trp1 lys2/lys2 | α/α | Sorbose selection from A658 (21) (this work) |
| 3148 | arg/arg/ARG/ARG ilv/ilv/ILV/ILV trp1/trp1/TRP1/TRP1 lys2/lys2/LYS2/LYS2 | a/a/α/α | 3316 × 3320 (31) |
| AF27 | ura3/ura3 his1::hisG/his1::hisG gal1::HIS1/gal1::URA3 | a/α | 9 |
a
Wild type refers to an absence of auxotrophic markers and known gene disruptions.
b
Strains constructed by growth on sorbose of parent strain.