FIG. 1.
EFV induces apoptotic cell death. (A) Jurkat T cells were seeded at 3 × 105 cells/ml and incubated with the indicated concentrations of AZT or EFV. Cell numbers were determined for every drug concentration every 2 days and expressed as a percentage of the untreated control cells. (B) Treated cells were analyzed using a Coulter EPIC Ultra flow cytometer by forward-scatter (FSC) (x axis) and side-scatter (SSC) (y axis) gating. (C) Percent LDH activity was measured after 24 h in comparison to that with camptothecin-treated cells. (D) Cell death was analyzed by flow cytometry with Annexin V-propidium iodide (PI) staining to distinguish apoptosis from necrosis. (E) Apoptosis was confirmed by histone DNA complex release.