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. 2005 Nov;73(11):7784–7787. doi: 10.1128/IAI.73.11.7784-7787.2005

FIG. 3.

FIG. 3.

RT-PCR analysis of P97 and P102 paralog gene transcription. A. Open reading frame numbers are shown for each open reading frame examined. Left lanes indicate RNA templates from BAL samples (R); right lanes, genomic DNA template controls (D). P97, intergenic, and P102 RT-PCRs are indicated at the top of the panel. The distance between the open reading frames in base pairs (bp) of each transcriptional unit are also shown. B. Controls. (Upper panel) Lane 1, no reverse transcriptase; lane 2, no RNA; lane 3, no primers; lane 4, P97 primers with cDNA; lane 5, P97 primers with genomic DNA. (Lower panel) These products are from RT-PCRs using RNA isolated from uninfected porcine lung tissue. Lanes 1 to 11 represent RT-PCRs with specific primer pairs for open reading frames mhp275, mhp272, mhp108, mhp107, mhp271, mhp280, mhp684, mhp683, mhp493, mhp385, and mhp384, respectively. Lane 12, P97-P102 primers. Lane 13 represents the pig cyclophilin positive control reaction.