TABLE 1.
Primers
| Primer | Function, open reading frame | Sequence (5′-3′) |
|---|---|---|
| For indicated P97 paralogs | ||
| mhp107 | ||
| 211-1020_U | Forward primer, mhp107 | GCGGATCCGATAATACTTATAATGCC |
| 211-1020_L | Reverse primer, mhp107 | ATGGATCCCTCTTTGGGAAATTGTTC |
| mhp271 | ||
| Mhp271-f | Forward primer, mhp271 | TTGGTTGGGTCTATTATTGG |
| Mhp271-r | Reverse primer, mhp271 | TAACTTTGCTAAATCATCGG |
| mhp280 | ||
| Mhp280-f | Forward primer, mhp280 | AAACAGCCGTGAAACTGC |
| Mhp280-r | Reverse primer, mhp280 | GCATTTTTGGATTCTTCTGG |
| mhp385 | ||
| 662-1020_U | Forward primer, mhp385 | ACGGATCCGATCAGCCACAGATTCAC |
| 662-1020_L | Reverse primer, mhp385 | ACGGATCCTAAAGCAAATAGTGATTCTGA |
| mhp493 | ||
| 545B-1020_U | Forward primer, mhp493B | ACGGATCCCAAAAATTAGCCACTTCC |
| 545B-1020_L | Reverse primer, mhp493B | ACGGATCCATCTATACTTCTTCAGTC |
| mhp684 | ||
| 347-1020_U | Forward primer, mhp682 | ACGGATCCAATCTTTTATCCGCTGGG |
| 347-1020_L | Reverse primer, mhp682 | ACGGATCCTGTTTGCTGATTTGAGGC |
| For indicated P102 paralogs | ||
| mhp275 | ||
| 033-1020_U | Forward primer, mhp275 | GCGGATCCATTTTTGGATGGAAACAAGAC |
| 033RT-r | Reverse primer, mhp275 | TGGGCTTACACCTTCTTTGGC |
| mhp272 | ||
| 036RT-f2 | Forward primer, mhp272 | GGCAAATGCTTTGATTTCC |
| 036RT-r2 | Reverse primer, mhp272 | GTTCTTTGCTGTGGACTTC |
| mhp108 | ||
| 210RT-f2 | Forward primer, mhp108 | GCAACAGCAAGTGGAACTGC |
| 210RT-r2 | Reverse primer, mhp108 | AAGTTGGTAAGCCGAGACTG |
| mhp683 | ||
| 348-1020_U | Forward primer, mhp683 | CGGGATCCCAAGCAAAAAATGAAAAAGAAG |
| 348RT-r | Reverse primer, mhp683 | TTTTACTTTAGCCGAAGAGGC |
| mhp384 | ||
| 663-1020_U | Forward primer, mhp384 | GCGGATCCACTAAATTATCACGAAGATCAC |
| 663RT-r | Reverse primer, mhp384 | TAGAACCATTGTAGCAGCCGG |
| For intergenic regions | ||
| P97-3′-f | Forward primer, mhp183 | GATTCAAAATCCGGTGATCC |
| P102-5′-r | Reverse primer, mhp182 | CTAGCTTGTTCTGTATTTCC |
| Mhp107-f | Forward primer, mhp107 | AAATGATGAAAATAGCCCG |
| Mhp108-r | Reverse primer, mhp108 | AGTTCCACTTGCTGTTGC |
| Mhp271-f | Forward primer, mhp271 | CCTGAAACTCCAAAAACAGAAG |
| Mhp272-r | Reverse primer, mhp272 | CAAAAGCCCGAACTAAGTCATAC |
| Mhp384-f | Forward primer, mhp384 | AGAGCATCAAAAGCGTTTAGTC |
| Mhp385-r | Reverse primer, mhp385 | TTCAGCAGCCAAGCCAGTAG |
| Mhp683-f | Forward primer, mhp683 | CCGCTTTTACTTCTTTATTAGCC |
| Mhp684-r | Reverse primer, mhp684 | GCCTCAAATCAGCAAACAAG |
| For controls | ||
| P97RT-f | Forward primer for control reaction, P97 | GGAAATTATGCCTATGAATTCG |
| P97RT-r | Reverse primer for control reaction, P97 | GTGCTCTGTTAGTTTCTAGTCC |
| P102RT-f2 | Forward primer for control reaction, pig cyclophilin | CCCGATGTGTTTTTAGATGG |
| P102RT-r2 | Reverse primer for control reaction, pig cyclophilin | CCTTTTCCTTTTCTATCGGC |
| cyclo-f | Forward primer for control reaction, pig cyclophilin | TAACCCCACCGTCTTCTT |
| cyclo-r | Reverse primer for control reaction, pig cyclophilin | TGCCATCCAACCACTCAG |