TABLE 2.
Changes in phosphorylation status of proteins in keratinocytes following exposure to bacteria
| Protein | Abbreviation | Phosphorylated epitope | % Of unstimulated controla |
|---|---|---|---|
| Cyclin-dependent kinase 1 | CDK1 | Y15 | 53 |
| Extracellular signal-regulated kinase 1 | ERK1 | T202/Y204 | 50 |
| Extracellular signal-regulated kinase 2 | ERK2 | T185/Y187 | 43 |
| Lyn (44) | Lyn (44) | Y507 | 175 |
| MAPK/Erk kinase 1/2 | MEK1/2 | S217/221 | 70 |
| Mitogen-activated protein kinase kinase 6 | MKK6 | S207 | 218 |
| p38 mitogen-activated protein kinase | p38 MAPK | T180/Y182 | 209 |
| p70 S6 kinase | S6Ka p70 | T421/T424 | 61 |
| Protein kinase B | PKBα (Akt1) | T308 | 49 |
| Protein kinase C α/β | PKC α/β | T638 | 53 |
| Raf (60) | Raf1 (60) | S259 | 19 |
| Raf (70) | Raf1 (70) | S259 | 67 |
| Retinoblastoma protein | Rb | S807/S811 | 59 |
| Type 1 protein phosphatase α | PP1α | T320 | 165 |
a
Keratinocytes were left unstimulated or stimulated for 10 or 30 min with E. cloacae; percent unstimulated control = 100% × normalized phosphorylation in a stimulated sample/normalized phosphorylation in an unstimulated control sample; a change of 25% or more is considered significant.