FIG. 2.
In vitro production of cytokines in GITR+/+ and GITR−/− mice during systemic C. albicans infection. GITR+/+ and GITR−/− mice were infected with 5 × 105 C. albicans cells and 6 days after infection spleen cells (A) or CD4+ T cells (B) were harvested. The cells were restimulated with heat-inactivated C. albicans (cell-C. albicans ratio, 1:10). Following 48 h incubation supernatants were harvested and Th1 (IL-12 p70 and IFN-γ) and Th2 (IL-4 and IL-10) cytokines were measured by enzyme-linked immunosorbent assay. Data represent means ± standard error of the mean of data from three independent experiments. *, P < 0.05 GITR−/− versus GITR+/+, according to Student's t test.