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Increase of IL-12 production after depletion of GITR+/+ CD4+CD25+ T cells. DCs from GITR+/+ and GITR−/− mice were cocultured with autologous CD4+ or CD4+CD25− T cells from infected mice in the presence of heat-inactivated C. albicans. IL-12 production was evaluated by DC intracellular staining. Data represent means ± standard error of the mean from three independent experiments. *, P < 0.05 column 2 versus 1 and 3 versus 1, according to Student's t test.
