FIG. 5.

Unprimed Treg cells from GITR^+/+ mice modulate production of IL-12 in DCs. DCs from C. albicans-infected GITR^+/+ and GITR^−/− mice were cocultured at the indicated ratio (DC:Treg) with autologous CD4⁺CD25⁺ Treg cells from noninfected mice in the presence of heat-inactivated C. albicans. IL-12 production was evaluated by intracellular staining on DCs. Data represent means ± standard error of the mean from three independent experiments. *, P < 0.05 GITR^−/− versus GITR^+/+, according to Student's t test.