FIG. 2.

TLR5 senses P. aeruginosa through flagellin in 293 cells. 293 cells were mock transfected or transfected with TLR2 or TLR5 DNA together with firefly and Renilla reporter genes as described in the legend to Fig. 1. Twenty-four hours after transfection, cells were incubated for 18 h with medium or 1, 10, or 100 CFU/cell of heat-killed PAK (A), PAK/fliC (B), or H. influenzae (C), and dual-luciferase assays were performed to measure induction of the luciferase activity in the cells. (D) Effect of PAK flagellin (1 μg/ml) or PAK/fliC mock flagellin preparation on TLR5 in 293 cells. The data are means ± standard errors for at least two independent experiments. Samples were compared to a control. One asterisk indicates that the P value is <0.05; two asterisks indicate that the P value is <0.001.