FIG. 6.

Effects of forskolin and PGE₂ on TNF-α and IL-10 mRNA levels. Cultured rat Kupffer cells were incubated for 30 min with forskolin (1 μM) or PGE₂ (1 μM) before the addition of LPS (1 μg/ml). Total RNA was isolated after 2 h, and TNF-α (A) and IL-10 (B) mRNAs were assessed by real-time RT-PCR and normalized for 18S RNA. Cytokine mRNA expression levels in LPS-treated cells are set to 100 in each individual experiment (black bars), and means ± SEM of the relative levels found in pretreated cells are shown. Three individual experiments are included. Unst, unstimulated; Ctr, control.