Skip to main content
. 2005 Nov;73(11):7290–7296. doi: 10.1128/IAI.73.11.7290-7296.2005

FIG. 7.

FIG. 7.

Expression of adenylyl cyclase 9 mRNA in Kupffer cells. (A) Total RNA from cultured Kupffer cells or whole liver was assessed by real-time RT-PCR using primers for adenylyl cyclase 9 (AC9) and adenylyl cyclase 6 (AC6) mRNA and normalized for 18S rRNA. The mean mRNA expression of each adenylyl cyclase mRNA in whole liver is set to 100, and relative expression levels in untreated rat Kupffer cells are shown. Liver samples from four individuals and Kupffer cells from three individuals are included. (B) Cultured rat Kupffer cells were incubated with LPS (1 μg/ml) or left untreated. Total RNA was isolated after 2 h, and adenylyl cyclase 9 (AC9) mRNA was assessed by real-time RT-PCR and normalized for 18S rRNA. Means ± SEM of samples from three individuals are shown. Mean adenylyl cyclase 9 mRNA expression in untreated cells is set to 100.