TABLE 1.
PCR primers used in this study.
| Name | Sequence (5′-3′) |
|---|---|
| nisRF | AATGATCGATAAACAATCGGAGGTa |
| nisKB | ACTAGTGGATCCCCCGGG |
| NZlisRF | GGGTTAGGCCCCATGGATAGAATACTAb |
| NZlisRB | AATGGTCTAGACGTCATGTACGCATc |
| lisRRTPCRF | CAGCGGTTGCTAATGATG |
| lisRRTPCRB | GATAACAGATCACGTGCG |
| HSOEA | CAGGTAGAGCGGAATTCATTGd |
| HSOEB | TTCATCGATTTCACTCTCCTTCCGTGTGTGTTAAGCGGATCCATa,e |
| HSOEC | ATGGATCCGCTTAACACACACGf |
| HSOED | CCTCTAGAGAATCTGCTTTTACCGTCc |
| PBP 1 | GCGACAAGGCCGGGGAAC |
| PBP 2 | CGGCGATTAGTCGCTTTG |
| CP 1 | AGACGCCCAGAACCGACTCCA |
| CP 2 | AATCGTACTCACGACGAGG |
| HKP 1 | CGGTACGGTCGGTTACTAT |
| HKP 2 | TGTCGCGCTTTTTTCACTCT |
a
Base changes made to incorporate a ClaI site are in boldface.
b
Base changes made to incorporate an NcoI site are in boldface.
c
Base changes made to incorporate an XbaI site are in boldface.
d
Base changes made to incorporate an EcoRI site are in boldface.
e
Overhang complementary to HSOEB primer is underlined.
f
Base changes made to incorporate a BamHI site are in boldface.