TABLE 2.
Oligonucleotide primers used in PCRs
| Gene and primer | Nucleotide sequence (5′-3′)a | Nucleotide coordinatesb |
|---|---|---|
| CdCDR1 | ||
| 1F | AATCTAGAATTATGTCAGATTCTAAGATGTC | −3 to +20 |
| 1R | ATTCTAGATTATTTCTTATTTTTTTTCTCTCTG | +4482 to +4506 |
| 2F | ATCCTGTTGGTTATGTGTTCG | +2071 to +2091 |
| 2R | GGGAAATCAACACTTCCAGTG | +2557 to +2537 |
| 3F | AGGAATTCCACTTGTGTGGGCATCAAACC | +121 to +101 |
| 3R | AGGAATTCCCAATGTTGGGATAATGCCAC | +969 to +989 |
| 4F | AGGAATTCAAACCTGGGCCACTTGGAAC | +2165 to +2146 |
| 4R | AGGAATTCCATTGTACAGTGAAAGATGGAG | +4373 to +4394 |
| 5F | ACCGCTCGAGCAGGTCTCATGATAGCATCC | +538 to +519 |
| 5R | ATCCCCGCGGAACTTGTTCTAGTTATTTGG | +4248 to +4267 |
| 6F | ATCCCCGCGGCAATACTTGTTGGTCAGTTGG | +3532 to +3552 |
| 6R | ACCGCTCGAGATATGGAGATGCTGGTCTGG | +1467 to +1448 |
| 7Rc | AATCTAGAGTTGATAATCAGAATCATTAGC | +421 to +400 |
| CdCDR2 | ||
| 8F | AATCTAGAAATATGAGTACTGCAAACACGTC | −3 to +20 |
| 8R | ATTCTAGATTATTTTTTCATCTTCTTTTCTCTATT | +4503 to +4467 |
| 9F | GCGAATTCAAACCTTGATATTCTTGAACTG | +97 to +76 |
| 9R | GCGAATTCGTTGGGATAATGCCACTAG | +989 to +971 |
| 10F | GCGAATTCTAATGCTTCTCTAACAGTAAGTGG | +2862 to +2840 |
| 10R | GCGAATTCCTATGGGACAATTGGCAATC | +3986 to +4005 |
| 7Rc | AATCTAGAGTTGATAATCAGAATCATTAGC | +418 to +394 |
| TEF3d | ||
| TEF3F | CGGAATTCCGATTGGTCCAAATGGTGCTGG | +2113 to +2132 |
| TEF3R | CGGAATTCCGATCTTGTTACCCATAGCATCG | +3012 to +3032 |
a
Restriction endonuclease recognition sequences are underlined. XbaI, 5′-TCTAGA; EcoRI, 5′-GAATTC; XhoI, 5′-CTCGAG; SacII, 5′-CCGCGG.
b
Nucleotide coordinates for CdCDR1 (accession no. AJ439073) or CdCDR2 (accession no. AJ439075) are as indicated, with the first base of the ATG start codon designated + 1.
c
The primer 7R is homologous to both CdCDR1 and CdCDR2.
d
Nucleotide coordinates of the TEF3 primers refer to the C. albicans TEF3 gene (accession no. Z12822).