Fig. 2.

The time course of events after culture for 1-6 days after electroporation with the green-positive, cyan-negative vector diagramed in Fig. 1 A. The vertical axis shows the percentage of sorted cells fluorescing green. Open bars indicate cells that produce nonfluorescing colonies when expanded. Gray bars indicate cells that produce green-fluorescing HAT-sensitive colonies; these are cells that have incorporated the GFP gene into their genomes nonhomologously. Black bars indicate cells that produce green-fluorescing HAT-resistant colonies; these are the cells in which the mutant gene has been corrected by homologous recombination. The numbers in the boxes show the percentages of sorted cells falling into the three categories indicated by the shading of the bars. The error bars show the standard errors of the results from four experiments. Note that, although a green-positive, cyan-negative vector was used in this experiment, sorting was only for green fluorescence. Note also that the vertical axis of the figure is interrupted and has two scales.