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. 2005 May 4;6:24. doi: 10.1186/1471-2121-6-24

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Copyright © 2005 Sagot et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Localization of several cellular structures or fusion proteins in cells with two daughters. A. Cells were untreated (right panel) or treated (left panel) with 100 μg/mL MPA for 4 hours. DIC (top lane), actin Alexa-phalloidin staining (middle lane) and DAPI staining (bottom lane) are shown. B. Localization of endogenous Tem1p-GFP (left panel), endogenous Lte1p-GFP (middle panel) and GFP-Cdc12p expressed from a centromeric plasmid under the control of its own promoter (right panel) in cells untreated (right of each panel) or treated for 4 hours with 100 μg/mL MPA (left of each panel). Only cells displaying two buds are shown in the case of MPA treatment. Bar: 2 μm.