FIG. 1.

Schematic representation of the entire and partially deleted SGI1. (A) XbaI (X), BamHI (B), and PvuII (P) restriction map was deduced from the DT104 DNA sequence released under EMBL accession no. AF261825. (B) Restriction map of the partially deleted SGI1 was experimentally determined. White boxes represent intI1, qacEΔ1, sul1, floR, tetR, tetA, ORF5, and ORF6 genes. The IS6100 position is indicated between two arrows. The shaded boxes represent gene cassettes inserted into the InC and InD integrons. Black boxes are the 59-base element sites, and DR-R and DR-L boxes represent the direct repeat junctions of SGI1 in the Salmonella chromosome (dotted lines). Thin bars represent Southern blot hybridization bands A, B, C, and D. Heavy lines represent PCR amplicons (PCR1 to PCR8). The positions of the intI1 gene and S014 probes are indicated.