TABLE 1.

In vitro antibacterial activities of DW286 and quinolones against clinical isolates

Microorganism(s) (no. of isolates) and compound	MIC (μg/ml)
	Range	MIC50	MIC90
QSSA (112)a
DW286	≤0.004-0.25	0.008	0.016
Ciprofloxacin	0.063-2	0.25	0.5
Gemifloxacin	0.008-0.125	0.031	0.063
Sparfloxacin	0.016-0.5	0.063	0.125
Trovafloxacin	0.016-2	0.063	0.125
QRSA (128)a
DW286	0.063-32	0.5	8
Ciprofloxacin	4->64	64	>64
Gemifloxacin	0.25->64	4	64
Sparfloxacin	1->64	16	>64
Trovafloxacin	0.25-64	8	64
S. epidermidis (53)
DW286	0.008-0.5	0.031	0.25
Ciprofloxacin	0.016->64	0.25	16
Gemifloxacin	0.016-4	0.063	2
Sparfloxacin	0.031-32	0.125	8
Trovafloxacin	0.031-4	0.063	1
S. pyogenes (34)
DW286	≤0.004-0.25	0.016	0.031
Ciprofloxacin	≤0.004-4	1	2
Gemifloxacin	≤0.004-1	0.063	0.25
Sparfloxacin	≤0.004-2	0.25	0.5
Trovafloxacin	≤0.004-0.5	0.125	0.25
Enterococcus faecalis (62)
DW286	0.031-16	1	4
Ciprofloxacin	0.25->64	4	>64
Gemifloxacin	0.125-64	4	32
Sparfloxacin	0.5->64	32	>64
Trovafloxacin	0.063-64	8	16
Enterococcus faecium (54)
DW286	0.063-16	4	8
Ciprofloxacin	0.5->64	16	>64
Gemifloxacin	0.063->64	8	16
Sparfloxacin	1->64	8	>64
Trovafloxacin	0.25-64	8	32
S. pneumoniae (30)
DW286	≤0.004-2	0.016	0.125
Ciprofloxacin	0.031-32	1	4
Gemifloxacin	≤0.004-8	0.063	0.5
Sparfloxacin	0.016-16	0.5	2
Trovafloxacin	0.031-4	0.25	0.5
E. coli (97)
DW286	0.016-16	0.063	0.5
Ciprofloxacin	0.008-8	0.031	0.5
Gemifloxacin	≤0.004-16	0.031	1
Sparfloxacin	≤0.004-32	0.016	0.5
Trovafloxacin	≤0.004-32	0.016	0.25
K. pneumoniae (78)
DW286	≤0.004-1	0.063	0.5
Ciprofloxacin	≤0.004-4	0.063	0.5
Gemifloxacin	≤0.004-2	0.063	1
Sparfloxacin	≤0.004-1	0.031	0.25
Trovafloxacin	≤0.004-0.5	0.063	0.25
K. axytoca (50)
DW286	≤0.004-0.25	0.016	0.125
Ciprofloxacin	0.016-0.25	0.031	0.125
Gemifloxacin	0.031-0.5	0.125	0.25
Sparfloxacin	0.016-0.5	0.031	0.125
Trovafloxacin	0.016-0.25	0.031	0.125
Citrobacter freundii (45)
DW286	0.016-16	1	2
Ciprofloxacin	≤0.004-4	0.125	0.5
Gemifloxacin	0.031-8	0.5	2
Sparfloxacin	0.031-8	0.5	2
Trovafloxacin	0.016-8	0.25	1
E. cloacae (65)
DW286	0.008-4	0.031	0.5
Ciprofloxacin	≤0.004-2	0.063	0.5
Gemifloxacin	0.031-4	0.25	2
Sparfloxacin	0.016-8	0.125	1
Trovafloxacin	0.016-8	0.125	2
E. aerogenes (30)
DW286	0.063-0.25	0.125	0.25
Ciprofloxacin	0.016-1	0.063	0.5
Gemifloxacin	0.063-0.25	0.063	0.25
Sparfloxacin	0.016-0.5	0.063	0.125
Trovafloxacin	0.031-0.5	0.125	0.25
Proteus mirabilis (45)
DW286	0.008-64	0.25	2
Ciprofloxacin	≤0.004-8	0.016	4
Gemifloxacin	0.016-64	0.125	0.5
Sparfloxacin	≤0.004->64	0.25	2
Trovafloxacin	0.008-32	0.125	0.5
Proteus vulgaris (13)
DW286	≤0.004-8	0.25	1
Ciprofloxacin	0.016-64	0.063	2
Gemifloxacin	≤0.004-64	0.063	0.25
Sparfloxacin	0.125->64	0.25	2
Trovafloxacin	0.063->64	0.125	2
Proteus rettgeri (11)
DW286	0.008-0.25	0.031	0.125
Ciprofloxacin	≤0.004-0.063	0.016	0.063
Gemifloxacin	0.016-0.125	0.031	0.063
Sparfloxacin	0.008-0.25	0.031	0.125
Trovafloxacin	0.008-0.125	0.031	0.063
Providencia stuartii (17)
DW286	0.125-2	0.25	1
Ciprofloxacin	0.031-1	0.125	1
Gemifloxacin	0.125-1	0.25	0.5
Sparfloxacin	0.063-1	0.125	0.5
Trovafloxacin	0.125-0.5	0.125	0.5
M. morganii (28)
DW286	0.031-16	0.25	0.5
Ciprofloxacin	0.008-4	0.016	0.5
Gemifloxacin	0.016-2	0.125	0.25
Sparfloxacin	0.008-2	0.25	0.5
Trovafloxacin	0.008-4	0.25	0.5
S. marcescens (81)
DW286	0.25-32	2	16
Ciprofloxacin	≤0.004-16	0.25	4
Gemifloxacin	≤0.004->64	0.5	8
Sparfloxacin	0.063->64	1	16
Trovafloxacin	0.063->64	0.5	4
Salmonella enterica serovar Typhimurium (41)
DW286	0.016-0.5	0.125	0.25
Ciprofloxacin	0.008-0.25	0.031	0.063
Gemifloxacin	≤0.004-0.5	0.063	0.125
Sparfloxacin	0.008-1	0.031	0.063
Trovafloxacin	0.008-0.125	0.063	0.125
S. enterica serovar Enteritidis (20)
DW286	≤0.004-1	0.125	0.25
Ciprofloxacin	≤0.004-0.125	0.031	0.063
Gemifloxacin	≤0.004-0.5	0.063	0.5
Sparfloxacin	≤0.004-0.5	0.063	0.125
Trovafloxacin	≤0.004-0.125	0.031	0.063
Shigella spp. (60)
DW286	≤0.004-4	0.016	0.125
Ciprofloxacin	≤0.004-1	0.016	0.031
Gemifloxacin	≤0.004-2	≤0.004	0.031
Sparfloxacin	≤0.004-1	0.016	0.063
Trovafloxacin	≤0.004-0.5	0.008	0.031
X. maltophilia (35)
DW286	0.25-8	1	4
Ciprofloxacin	0.5-8	2	8
Gemifloxacin	0.5-2	1	1
Sparfloxacin	1-4	1	2
Trovafloxacin	0.5-2	0.5	2
A. calcoaceticus (35)
DW286	0.063-8	0.25	2
Ciprofloxacin	0.016-16	0.5	1
Gemifloxacin	0.031-8	0.25	4
Sparfloxacin	0.016-4	0.25	2
Trovafloxacin	0.031-8	0.25	2
H. influenzae (20)
DW286	≤0.004-0.063	0.004	0.008
Ciprofloxacin	≤0.004-0.5	0.004	0.016
Gemifloxacin	≤0.004-0.031	0.004	0.016
Sparfloxacin	≤0.004-0.063	0.004	0.063
Trovafloxacin	≤0.004-0.031	0.004	0.031
P. aeruginosa (70)
DW286	≤0.004->64	4	32
Ciprofloxacin	≤0.004->64	0.25	2
Gemifloxacin	≤0.004->64	2	8
Sparfloxacin	≤0.004->64	1	8
Trovafloxacin	≤0.004->64	0.5	4

^aQSSA, quinolone-susceptible S. aureus (ciprofloxacin MIC ≤ 2 μg/ml). For QRSA, ciprofloxacin MIC ≥ 4 μg/ml.