FIG. 1.

Effect of SLS (A and B) and LS (C and D) on the infectivities of HSV-1 strain F (○) and HSV-2 strain 333 (•) on Vero cells. In a first set of experiments, confluent cell monolayers were inoculated with viruses pretreated with PBS (control) or with PBS containing increasing concentrations of surfactants for 1 h at 37°C (A and C). In a second set of experiments, surfactants and viruses were added simultaneously to cells without any pretreatment period (B and D). In both cases, the plates were immediately centrifuged (750 × g for 45 min at 20°C) to allow virus adsorption. Unbound viruses were removed by aspiration. Cell sheets were overlaid with EMEM-2% FBS containing 0.6% SeaPlaque agarose and incubated for 48 h at 37°C. Infectivity was expressed as the percentage of PFU compared with that of control cells. Results are the means ± standard deviations (error bars) of three independent experiments.