FIG. 3.

(A) Effect of SLS (○) and LS (•) on the attachment of [³H]methyl thymidine-labeled HSV-2 strain 333 to Vero cells. Viruses were first pretreated with PBS plus 1% BSA (control) or with PBS-1% BSA containing increasing concentrations of SLS or LS for 1 h at 37°C. Confluent cells were then incubated with pretreated viruses for 1 h at 4°C. Cells were washed three times with PBS and lysed, and radioactivity associated with cell lysates was determined by liquid scintillation counting. Binding was expressed as the percentage of counts per minute in the control cell lysates. Results are the means ± standard deviations (error bars) of three independent experiments. (B) Correlation calculated between inhibition of viral infectivity (Fig. 1C) and inhibition of viral attachment (Fig. 3A) data obtained following pretreatment of HSV-2 strain 333 with increasing LS concentrations for 1 h at 37°C.