FIG. 5.

Effect of SLS (○) and LS (•) on the rate of penetration of HSV-2 strain 333 in Vero cells. Virus was first adsorbed to the confluent cell monolayer after a 2-h incubation period at 4°C. After removal of unbound viruses, cells were shifted to 37°C to allow penetration into cells. At selected times after the temperature shift (0, 15, 30, and 60 min), cells were treated for 1 min with PBS (control [□]) or PBS containing 300 μM SLS or 500 μM LS prewarmed at 37°C. Cell sheets were overlaid with EMEM-2% FBS containing 0.6% SeaPlaque agarose and incubated for 48 h at 37°C. Results were expressed as the number of PFU. Values are the means ± standard deviations (error bars) of three independent experiments.