FIG. 1.
Effects of ethanolic extracts from 10 Chinese herbs on HSV-1 replication in Vero cells determined by plaque reduction assay. Vero cells (3.5 × 105/dish) were incubated with 100 PFU of HSV-1 and 10 μM acyclovir, 100 μg of each extract/ml (A), or the indicated concentrations of L. sinense extracts (B). After adsorption for 1 h, 1% methylcellulose was added to each well. On the fifth day p.i., the virus plaques formed in Vero cells were counted by crystal violet staining. Each bar represents the mean of three independent experiments. VL, Ventilago leiocarpa, ER, Ecdysanthera rosea, EU, Ecdysanthera utilis, HL, Hippobroma longiflora, AB, Ardrisia brevicaulis, SD, Selaginella delicatula, LS, L. sinense, AJ, Ardrisia japonica, AV, Ardrisia violacea, AE, Andendron benthamianum.