FIG. 4.

Effects of Sam B on HSV-1 replication and Vero cell viability and growth. (A) Inhibitory effects of 10 μM acyclovir and indicated concentration of Sam B on HSV-1 replication were determined by plaque reduction assay. Each bar represents the mean of three independent experiments. (B) Vero cells (3.5 × 10⁵ in 25-cm² flasks) were treated with medium, 0.1% DMSO, or 25, 50, or 100 μM Sam B for 5 days. Then total, viable, and nonviable cells were counted after staining with trypan blue. Each bar represents the mean of three independent experiments. (C) Vero cells (5 × 10³/well of a 96-well plate) were treated with Sam B at the indicated concentrations or were treated without Sam B for 5 days. Then tritiated thymidine, tritiated uridine, or tritiated leucine was added to each well (1 μCi/well) to detect cell growth. After a 16-h incubation, the cells were harvested by an automatic harvester and then radioactivity was measured by scintillation counting. Each bar represents the mean of three independent experiments.