FIG. 5.

Kinetics of inhibition of HSV-1 replication by Sam B. (A) Vero cells (5 × 10⁶) were infected with HSV-1 at an MOI of 3 in the presence (▾) or absence of acyclovir (10 μM) or Sam B (25 μM). Treatments were as follows: Sam B was added at the same time as HSV-1 (▿), Sam B was present after the adsorption period (□), and Sam B was present for 2.5 h and removed before the adsorption phase by washing the cells with medium three times (▪). Then cell supernatants were collected at 0, 2, 4, 6, 8, 16, 24, and 36 h p.i. and the viral titers were determined by a plaque forming assay. Each point represents the mean of three independent experiments. (B) Vero cells (5 × 10⁶) were infected with HSV-1 (MOI, 3), and Sam B (25 μM) was added at the indicated times. Cell supernatants were collected at 25 h p.i., and HSV-1 titers were determined as described in Materials and Methods. Each bar represents the mean of three independent experiments.