FIG. 8.

Effects of Sam B on DNA polymerase gene transcription in Vero cells detected by Northern blot analysis. Vero cells (5 × 10⁶) were infected with HSV-1 (MOI, 3) or were not infected in the presence or absence of Sam B (25 μM). (A) Total cellular RNA was isolated from the cells at 6 h p.i. and analyzed in 6.66% formaldehyde-agarose gel and hybridized with digoxigenin-labeled DNA polymerase cDNA or β-actin cDNA. Lanes 1 and 4, HSV-1-infected cells treated with or without DMSO; lane 2, HSV-1-infected cells treated with Sam B; lane 3, uninfected cells. (B) Each exposed band was quantitated by densitometer, and the ratio of DNA polymerase mRNA to β-actin mRNA was calculated. Each bar is the mean of three independent experiments.